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The Past · Antony van Leeuwenhoek (1675)-Microorganism was first seen · Louis Joblot (1718) & Louis Pasteur (1860)-Organism arose by spontaneous generation was wrong · Robert Koch (1877) -Easy microscopic staining using aniline dye· Robert Koch (1881) -lsolate pure culture of bacterial on a solid culture medium What is Infection?· The invasion of a host bodily tissues by disease causing organisms, their multiplication, and the reaction of host tissues to these organisms and the toxins they produce.