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Purpose:The main barrier of cell therapy is seeding cells will lose multi-differentiation potential and their surface markers will change after long term culture in vitro.We developed 3D perfusion system to advance long term cell culture strategy to maintain seeding cells multiplication capacity and multi-differentiation potential.To further explore the mechanism of 3D perfusion system promoting cell proliferation and osteogenesis, we performed gene array to analyze gene expression profiles changes after cells were cultured in 3D perfusion system.