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Nanopore technology as such an emerging research area attracts most attentions of scientists and chemists recently.These nanopores are successfully used as sensors to monitor ion currents and forces via different electrochemistry systems,which were known as ion current rectification ( ICR) and/or resistive pulse[1].Also they can be used as a medium to examine the signals of biological processes,such as single molecules detection[2].DNA segments sequencing detection[3-4].Herein.we have shown a new class of creative idea that research on the formation of liposomes based on glass-based nanopores.The size and shape of the nanopores were characterized by optical microscopy,scanning electron microscopy ( SEM) with an opening diameter in the tip of nanopore about 200 nm.The electrochemical characteristics of the nanopores were characterized by linear sweep voltammetry ( LSV) with 0.I mol/L KCl solution inside and outside the pore.Furthermore.we have demonstrated the effects of pH,electrolyte,solution concentration,or applied voltage on the size and the shape of liposomes during formation.In addition.we try to describe the dynamics of the liposomes formation through nanopores,which was different from traditional formation of liposomes on the surfaces of the substrates[5].