【摘 要】
：
目的:观察用聚乙烯亚胺(polyethylenimine,PEI)包被的SPIO(PEI-SPIO)作为基因载体时对细胞的毒性以及将REST-siRNA、SHH-siRNA共转染hADSCs后其分化为胰岛前体细胞的能力.方法:台盼蓝染色、MTT、免疫荧光、Real-time PCR、Western-blot、普鲁士蓝染色实验结果一、hADSCs的分离和培养原代培养hADSCs 24小时后,少量细胞
【机 构】
：
中国医科大学细胞生物学卫生部重点实验室干细胞与再生医学研究室,沈阳110122 中国医科大学附属第
【出 处】
：
首届全国组织修复与再生医学大会暨国际组织修复与再生医学学术研讨会
论文部分内容阅读
目的:观察用聚乙烯亚胺(polyethylenimine,PEI)包被的SPIO(PEI-SPIO)作为基因载体时对细胞的毒性以及将REST-siRNA、SHH-siRNA共转染hADSCs后其分化为胰岛前体细胞的能力.方法:台盼蓝染色、MTT、免疫荧光、Real-time PCR、Western-blot、普鲁士蓝染色实验结果一、hADSCs的分离和培养原代培养hADSCs 24小时后,少量细胞贴壁.传代细胞很快贴壁,细胞形态较均匀一致,呈长梭形均匀贴壁分布.二、hADSCs细胞表面抗原标志物流式细胞学鉴定流式细胞仪(Flow cytometer,FCM)检测结果显示,hADSCs不表达CD34,主要表达CD105和CD90.表明所分离的hADSCs不包含造血干细胞及成熟的造血细胞,是一群高表达间质细胞表面分子的细胞..
其他文献
Objective To investigate the role of human mesenchymal stem cells (hMSCs) in regulating the chemosensitivity of breast cancer cells and to dissect the underlying molecular mechanism.Methods The human
Background: Cell proliferation and turnover are fueled by stem cells.In a previous study, we demonstrated that rat eccrine sweat glands contained abundant bromodeoxyuridine (BrdU)-label retaining cell
Prior exposure to lipopolysaccharides (LPS) induces host reprogram state of repressing inflammatory responses, however, the molecular mechanisms remain elusive.Exosomes play a pivotal role in cell-to-
目的:人脐带间充质干细胞(human umbilical cord-derived mesenchymal stem cells,hUC-MSCs)对肿瘤有趋化性,而且免疫原性低、增殖力强、取材方便、来源广泛,具有广阔的应用前景.IL-29又名IFN-?1,具有抗病毒、抗肿瘤和免疫调节效应.本研究利用hUC-MSCs作为靶向运载工具,构建IL-29过表达慢病毒载体(pBpLV-IL29),探究过表
Background: Genetic modified Schwann cells (SCs) over-expressing neurotrophic factors (NFs), especially those with multiple NFs over-expression, hold great potential to promote nerve regeneration.Thus
The impairment of wound healing in diabetic patients is an important clinical problem.Proper keratinocyte migration and proliferation are the crucial steps during re-epithelialization, and these steps
Background: High glucose can induce apoptosis in a variety of cells.In vivo high glucose is often complicated by the sympathetic deactivation.Sympathetic nervous system can regulate various bone marro
The isolation of some specific kind of cell type from bone marrow stromal cells is a main obstacle in the research of the mechanism and process of bone marrow hematopoiesis, for the reason that MSCs a
Objective: Type 2 diabetes mellitus (T2D), one of the most widespread endocrine disorders, is characterized with insulin resistance and relatively insulin deficiency.Insulin resistance, typically pres
Purpose:Mouse lung multipotent stem cells (MLSCs) could regenerate many different lung components including bronchioles, alveoli, and pulmonary vessels.But the research of MLSCs were limited due to it