【摘 要】
：
The aim of this study is to shed light on the genetic diversity and phylogenetic status of Diandongnan buffalo (Bubalus bubalis).Through polymerase chain reaction (PCR) and direct sequence,29 mtDNA D-
【机 构】
：
National Beef Cattle Improvement Center of China, Yangling Shaanxi712100, China;Yunnan Academy of Gr
【出 处】
：
2011中国肉牛选育改良与产业发展国际研讨会
论文部分内容阅读
The aim of this study is to shed light on the genetic diversity and phylogenetic status of Diandongnan buffalo (Bubalus bubalis).Through polymerase chain reaction (PCR) and direct sequence,29 mtDNA D-loops were sequenced.The lengths of mtDNA D-loops ranged from 910~917 bp.Combined with five mtDNA D-loops of Diandongnan buffalo from GenBank, 20 haplotypes were defined showing that haplotype diversity, nucleotide diversity and average number of nucleotide differences were 0.877±0.053, 0.01091±0.00465 and 9.865±21.419, respectively.Mismatch distribution analysis showed that extant Diandongnan buffalo population lasts a stable population with abundant diversity.A N-J tree was constructed by gathering the mtDNA D-loop sequences involving in Egyptian, Indian, Mediterranean, Brazilian, and Chinese buffalo breeds, which revealed two distinct clades (swamp-type and river-type clades), and all the Diandongnan buffalo clustered together with swamp-type clade and split from river-type buffalo including Indian, Egyptian and Mediterranean buffalo.Diandongnan buffalo further split two sub-clades, SW-A and SW-B in accordance with the cases of other Chinese buffalo breeds.Despite Yunnan province was located in the southwest border of China, a historic commercial entrance, no Indian river-type mitochondrial haplotypes were found in Diandongnan buffalo indicating that Diandongnan buffalo hadn,t yet been undergone gene introgression from Indian buffaloes and Diandongnan buffalo belongs to pure swamp-type without Indian river-type haplotypes supporting the hypothesis of China or Southeast Asian origin.
其他文献
Agriculture Environmental Renewal Canada (AERC) Inc.is a Canadian research and development seed company specialized in the development of forage, silage and grain hybrids of sorghum (Sorghum bicolor,
Canadian Forage Sorghum Hybrid 30 (CFSH 30) produces better yields with excellent forage quality over a wide range of growing conditions.This hybrid has been tested widely across several locations in
The maturing sperm cells discard the majority of their cytoplasm during the final stages of spermatogenesis and lose some of their defense-enzymes.The purpose of the present study was to investigate t
This experiment was conducted to investigate the effects of different grain-dregs dietary energy density on growth performance and intramuscular fat deposition in beef cattle.Thirty finishing Simmenta
To study the effect of traditional Chinese medicine compound on the grown performance,blood physiological parameters and nutrient digestibility of beef carles under high temperature.Twenty-seven jinji
The red soils region of southern China belongs to the subtropical monsoon climate which characterized by sufficient solar radiation, abundant heat and plentiful rainfall and the four seasons are disti
The objective of the present experiment was to evaluate the effect of different dietary energy density of Pomace and Pulp on the Blood index, the Content of Intramuscular Fat,Hormone-sensitive lipase
Three Luxi yllow cattle (350±9 kg) were used in 3×3 Latin Square Design to evaluate the influence of varying ruminally degradable protein (RDP) to ruminally undergradable protein (RUP)ratio on ruminal
During the last decade, emergence of sequence data related to domestic animals like cattle,chicken, and goat laid support in favor of multiple domestication against the single domestication event.In t
The aim of this study was to evaluate the effect of different source of starch on in vitro fermentation characteristics.The experiment contained four trials which were basal diet (B), basal diet plus