QUANTIFICATION OF PEANUT ALLERGEN ARA H 2 USING RABBIT POLYCLONAL ANTIBODY*

来源 :第十四届世界食品科技大会 | 被引量 : 0次 | 上传用户:blusky
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  Peanut allergy can have serious reactions to trace of peanut allergens and often need extreme measures to avoid potential contact with these allergens.Therefore,it is necessary to develop a quick,accurate and sensitive method to detect the trace Ara h 2,the major peanut allergens,in foods.To develop a competitive inhibition ELISA for quantification of Ara h 2,a standard Ara h 2 allergen was derived from the purification of crude peanut proteins extract using anion-exchange chromatography,and a specific rabbit serum was raised against Ara h 2 from Japanese rabbits immunized with routine method.
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Abstracts:Peanut allergy is one of the most serious food allergies on account of their widespread use as ingredients in many food products.Ara h 2 is one of paramount allergens in peanut,which has bee
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