Objective: The signal transducer and activator of transcription–3(Stat3)mediates the expression of a variety of genes in response to cell stimuli,and thus plays a key role in many cellular processes such as cell apoptosis and cell cycle.In addition,STAT3 is dysregulated in most tumors,such as multiple myeloma,leukemia,breast cancers,lung cancers and so on,which is closely associated with tumorigenesis.Multiple myeloma(MM)is a fetal hematological malignancy due to lack of effective drugs in the treatment of MM.This study is intendedto find novel anti-myeloma STAT3 inhibitors by a high-throughput screening and thread light on its anti-myeloma mechanism.