Background and purpose: APT102 is a recombinant protein which exhibited more than 4-fold higher ADPase activity and sustained-50-fold longer plasema half-life as compared to the native apyrase.The aim of our study is to investigate the effect of APT102 on myocardial reperfusion injury in rats and evaluate it with 7.0T MRI.Materials and methods: Acute myocardial I/R injury was induced in 13 female Sprague-Dawley(SD)rats,by ligating the left anterior descending(LAD)coronary artery for 60 minutes,and was followed by reperfusion.These 13 SD rats were divided into 2 groups(G1 and G2)randomly.Rats in G1 were treated with APT102(0.6mg/kg)at the time of reperfusion and 24 hours,48 hours,72hours,4 days,5 days,6 days after reperfusion.As a contrast,G2 rats treated with saline.All rats were scanned at a 7.0T MRI(BRUKER BIOSPEC 70/30,Ettlingen,Germany)24 hours,48hours,7 days after perfusion,using T2 mapping、T1 ρ mapping and late gadolinium enhancement imaging(LGE).T2 mapping was used to access edema,area at risk(AAR)and intramyocardial hemorrhage; T1 ρ mapping was used to access myocardial fibrosis; LGE was used to calculate left ventricular eject fraction(LVEF)and infarct size.Gross sections of the left ventricular myocardium and corresponding hematoxylin and eosin staining and masson stain were assessed for myocardial infarction and myocardial fibrosis.Results: At present,we have 3 rats in G1 and 7 rats in G2,3 rats died,two of it died during the surgery,one during the sccaning.Rats in G1 have a lower infact size(infarct size volume/left ventricular volume)(n=3; 20.28%±3.92%vs.n=7; 35.81%±10.55%,p=0.043)and higer LVEF(n=3; 67.40%±7.00%vs.n=7; 51.21%±4.45%,p=0.02)compared with rats in G2 24hours after reperfusion.The research willed be continiued,we will accuqaire other indexes like AAR/LV,INF/AAR,volume of hematoma,and data 48hours\7 days after reperfusion.Conclusion: APT102 has benifite on acute myocardial ischemia/reperfusion injury.