低辐射污染区域盐爪爪根际细菌群落多样性初步分析

来源 :第七届全国微生物资源学术暨国际微生物系统与分类学研讨会 | 被引量 : 0次 | 上传用户:mixiaoya2008
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  根际微生物作为土壤-植物间物质交换活跃界面的重要的成员之一,参与和担负着根际重要的生理过程,维持着根际的生态平衡,在与植物根系相互影响,相互促进,协同进化,形成了陆地最重要的生态系统之一。盐爪爪作为我国西北某辐射污染区域典型的盐生植物,具有吸收和转移多种放射性物质的能力,在盐碱土壤改良和生态重建中,特别是核素污染治理中具有重要应用价值。相关研究证明,盐爪爪生长过程中根际存在离子富集作用,尤其具有较高富集钠、钾离子的能力,在其根际形成"离子岛效应"。然而,目前有关盐爪爪根际微生物的群落和组成以及在根际微生物其群落组成分布和演替,根际微生物在矿物离子富集,尤其是在辐射污染环境下,放射核素吸收和转移过程中作用,知之甚少。本研究,对某一放射性核素沉降区低辐射污染区域中盐爪爪根际土壤进行样品采集,通过菌株的分离和筛先,共获得各类细菌100余株,通过16S rDNA序列分析,确定其涉及Acinetobacter、Pseudomonas、Ensifer、Luteimonas、Oceanobacillu、Bacillu、Planmicrobium planococcu、Gracibacillus、Massillas、Psychrobacter、Prolinoborus、Brevibacterium、Iodobacter、Herbaspirllum、Halomonas 等17个属40余个种,其中多数菌株为革兰氏阳性菌,Pseudomonas和Bacillus为主要的菌群,其中Luteimonas和Prolinoborus属在此前辐射污染区细菌多样性研究中从未发现。研究过程中发现3个潜在新种,B1、B98和B106分别归属于Herbaspirllum、Halomona和Acinetobacter,其于相关模式菌最大同源性均小于98%,初步证明盐爪爪根际微生物存在丰富的群落多样性,且蕴藏着为数可观的新物种资源。
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