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Objective To develop a direct, non-invasive method for the easy identification and localization of the recurrent laryngeal nerve and the tubercle of Zuckerkandl.Methods At first, a study group of 14 persons without any prevailing medical conditions were chosen.Then ultrasound imaging is performed on both side of the neck in each individual.Both the left and right recurrent laryngeal nerve is identified and its distance is calculated from the skin,wternocleidomastoid muscle, mid line of the trachea and the carotid artery.Diameter of the nerve is also calculated.The tubercle of Zuckerkandl is searched for in the posterolateral aspect of the thyroid lobe.Results Distance of left RLN from skin is (14.68±1.62)mm, from sternocleidomastoid muscle is (8.37±0.36)mm, from carotid artery is (12.25±0.54)mm, from mid point of the trachea is (7.93±0.2)mm.Diameter of the left RLN is (1.13±0.14)mm.Distance of right RLN from skin is (14.43±1.89)mm,from sternocleidomastoid muscle is (8.76±0.54)mm, from carotid artery is (12.72±0.38)mm, from mid point of the trachea is (8.05±0.21)mm.Diameter of the right RLN is 0.86±0.1.Another interesting finding is the identification and location of the tubercle of Zuckerkandl in the study group persons.Conclusion This study represents an initial attempt to identifiy and localize the recurrent laryngeal nerve on both right and left side of the neck by means of high frequency ultrasound imaging.This direct and noninvasive method for the localization of the nerve would be very beneficial in surgical practice.This can be used to easily visualize the nerve before performing surgical operations, intraoperatively or as follow up after surgeries to look for anomalies.The tubercle of Zuckerkandl is appropriately identified and is of great value in surgical practices, because previously it was also at a big risk of getting damaged during performing operations.