Ethanol is one of risk factors associated with osteonecrosis,it has been demonstrated that ethanol induces adipogenesis,decreases osteogenesis in bone marrow stroma cells and produces intracellular lipid deposits,resulting in the death of osteocytes.In this approach,we isolated human bone marrow stroma cells and triggered for different differentiations.These cells could be inducted for osteogenesis,adipogenesis and chondrogenesis.We also evaluated cell surface markers of isolated human bone marrow stroma cells that were found to express CD29,CD49d,CD62 CD90,CD105/SH2,SH3,CD133,and CD166,but not CD31,CD34,CD45,or CD56.We demonstrated that ethanol decreases the expressions of osteogenic genes,but increased adipogenic genes expressions.Moreover,we found that ethanol decreases the beta-catenin-dependent canonical Wnt signaling pathway related gene expressions,including Wnt 3a and LRP5 genes.Interestingly,ethanol also diminishes the intra-nuclear translocation of β-catenin in human bone marrow stroma cells.Therefore,these results indicate that ethanol might decrease osteogenic gene expressions through Wnt signaling pathway.