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Drought stress is a major factor that limits crop yield.Maize (Zea mays L.) is the first important crop in china and third important crop worldwide because of its economic importance and nutritional value.But, it is considered as one of the most drought sensitive crops.It is thus imperative to study the mechanism of resistance and adaptation to drought stress.As an important secondary messenger molecule, Nitric oxide (NO) plays an important role in the regulation of stress responses.In view of our previous findings on demonstrating the role of NO in mediating maize seedlings responses to drought stress, in the experiment, we investigated the proteome pattern of NO-pretreated maize seedlings under drought stress to decipher the molecular mechanisms underlying NO-induced tolerance to drought stress.