【摘 要】
：
Agrobacterium tumefaciens GW4 is a heterotrophic,aerobic arsenite [As(Ⅲ)]-oxidizer.Different with other well-recognized heterotrophic As(Ⅲ)-oxidizing stra
【机 构】
：
华中农业大学农业微生物学国家重点实验室,生命科学技术学院,武汉,430070
【出 处】
：
第十八次全国环境微生物学学术研讨会
论文部分内容阅读
Agrobacterium tumefaciens GW4 is a heterotrophic,aerobic arsenite [As(Ⅲ)]-oxidizer.Different with other well-recognized heterotrophic As(Ⅲ)-oxidizing strains,the growth of strain GW4 improves with the addition of As(Ⅲ).The generated arsenate was suggested to involve in the synthesis of polar lipids.In this study,proteomics,qRT-PCR and analysis of ATP,NADH and metabolites were employed to determine the cellular responses to As(Ⅲ).Twenty-seven differentially expressed proteins/genes were identified and primarily classified as involved in the metabolism of As,Pi,carbohydrate,lipid,energy and motility.The cellular concentrations of NADH and ATP increased with As(Ⅲ).
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