目的：筛选和制备高效价的埃博拉GP蛋白单克隆抗体,验证单克隆抗体在ELISA, Western blot,细胞免疫荧光实验、细胞免疫化学实验中的应用。方法：设计靶肽,筛选抗体表位,人工合成埃博拉GP蛋白多肽。用多肽配合博奥龙公司生产的细胞因子佐剂免疫小鼠,通过5周的免疫法,制备免疫小鼠脾脏淋巴细胞,采用细胞融合技术,与SP2O细胞融合,制备杂交瘤细胞。
目的：以非酒精性脂肪性肝病(NAFLD)SD大鼠为模型，探讨Kupffr细胞在NAFLD发展过程中激活方式的变化及对大鼠机体造成的影响。方法 分别选取3周龄及8周龄大鼠各40只，幼鼠随机分成模型组和对照组，成年组亦是。各组分别为20只。
目的：了解IL-1β、IL-33、IL-1RA在CIA小鼠模型血清中的表达情况，及shRNA特异性干扰沉默HIF-1α对其在CIA小鼠血清及关节滑膜组织中表达的变化。方法：选用20只6-8周龄SPF级DBA/雄性小鼠，分成完全对照组及CIA对照组、阴性病毒对照组、HIF-1α干扰组，应用ELASA法检测治疗前后血清中IL-1β、IL-33、IL-1RA的表达；根据体内转染HIF-1α减低表达慢病毒
Objective: To explore the clinical diagnosis value of cytokines in different of infections.Methods: The serum levels of IL-2,IL-4,IL-6,IL-10,IFN-γ and TNF-α were measured by flow cytometry.Results: Co
Severe neurological complications in Henoch-Schonlein purpura (HSP) are rare,let alone cerebral hemorrhage.Here we deseribe a 5-year-old girl with HSP presented with acute onset of headache and confus
目的：评估重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白(rhTNFR∶Fc)在儿童幼年类风湿性关节炎(JRA)中治疗的安全性及有效性。方法：回顾性分析2011年至2015年于我院儿童肾内科确诊JRA并进行rhTNFR∶Fc治疗的临床病例资料16例(治疗组)，并与同期进行甲氨蝶呤治疗的JRA患儿(对照组)进行比较安全性及有效性比较。
Introduction: Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) in children arc rare,but potentially life-threatening mucocutaneous reactions.The exact pathogenesis of SJS/TEN in chi
Objectives: To investigate the association between level of serum uric acid in children with diabetic ketoacidosis (DKA) Methods: Eighteen DKA children were divided into two groups: those with hyperur
Objective : To evaluate the clinical significance of osteopontin (OPN) in serum and joint fluid in juvenile idiopathic arthritis (JIA).Methods :39 children with JIA in acute phase or remission phase a
Objective: To explore into the values of applying MRI fat saturation sequence checking into juvenile arthritis.Methods: Select 1,131 juvenile arthritis cases.1,601 had symptomatic arthritis.MRI normal