JS399-19, 2-cyano-3-amino-3-phenylancryic acetate, is a novel cyanoacrylate fungicide discovered and patented by the Jiangsu Branch of the National Pesticide Research & Development South Center of China.This paper presented the baseline-sensitivity of Fusarium verticillioides to the fungicide JS399-19 and evaluated the impact of JS399-19 on the germination of rice seeds and its efficacy in controlling rice bakanae disease by seed treatment.The EC50 values for JS399-19 inhibiting mycelial growth of wild-type F.verticillioides isolates ranged from 0.162 to 0.559 μg/ml (a 3.5-fold difference between the lowest and highest value), and the average (± SE) EC50 value was 0.316 ± 0.072 μg/ml.The sensitivity curve was unimodal over the sensitive range and the sensitivity data could be used as a baseline for monitoring any future changes in sensitivity to JS399-19 by F.verticillioides populations.Germination of healthy seeds was greater than 77% for all the selected cuhivars, and germination was unaffected by treatment with JS399-19.Treatment of infected seeds (harvested from diseased plants) with all concentrations of JS399-19 in this study significantly decreased the abnormal elongation and damping-off of seedlings in plastic bottles in the greenhouse, indicating that JS399-19 exhibited excellent efficacy in controlling rice bakanae disease, and was safe to rice seeds and could be used as a disinfectant of rice seeds infected by F.verticillioides.The results also suggested that the F.verticillioides isolates exhibited little sensitivity shift to this fungicide after once seed treatment.If properly applied, JS399-19 might be used as a major fungicide or a good companion fungicide for the control of rice bakanae disease in China.