The Aurora kinases are a family of serine/threonine kinases that interact with components of the mitotic apparatus and serve as potential therapeutic targets in oncology.[1,2] Here we synthesized 4 series of 2,4-diaminopyrimidines from 5-substituted 2,4-dichloro-pyrimides and evaluated their biological activities,including antiproliferation,inhibition against Aurora kinases and cell cycle effects.