【摘 要】
:
The method of determination of oridonin in the crude drugs by HPLC with a new phenylalanine substituted calyx [4] arene bonded silica stationary phase(PHE-Cx4)was establishment.The separation was perf
【机 构】
:
郑州大学化学与分子工程学院,郑州450052
【出 处】
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全国生物医药色谱及相关技术学术交流会(2012)
论文部分内容阅读
The method of determination of oridonin in the crude drugs by HPLC with a new phenylalanine substituted calyx [4] arene bonded silica stationary phase(PHE-Cx4)was establishment.The separation was performed on PHE-Cx4 column with a mobile phase of acetonitrile-H2O (20:80,v/v).The flow rate was 1.0 mL/min and the detecting wavelength was 239 nm.The liner range was 0.6~60 μg/mL (r=1),The detection limit was 0.15 μg/mL,The average recovery was 99.2%,RSD=2.2%(n=6).The oridonin can be well separated and detected in the crude drugs with the sample pretreatment according to the method of "Chinese Pharmacopoeia".The method is simple,sensitive,accurate and precise.
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