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Aim: To investigate the effect of TAT-tCNTF on injury of human umbilical vein endothelial ceils (HUVEC) induced by cytochalasin D (CD) and the action mechanism.Methods: TAT-tCNTF and CD affected cell viability by CCK-8.Using fluorescence microscope we observed changes of F-actin resulted by CD and TAT-tCNTF.