【摘 要】
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目的 探讨15-Deoxy-△12,14-prostaglandin J2(15d-PGJ2)对小鼠单核巨噬细胞中巨噬细胞移动抑制因子(macrophage migration inhibitory factor, MIF)表达的调节及机制.方
【机 构】
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首都医科大学细胞生物学系,北京100069
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目的 探讨15-Deoxy-△12,14-prostaglandin J2(15d-PGJ2)对小鼠单核巨噬细胞中巨噬细胞移动抑制因子(macrophage migration inhibitory factor, MIF)表达的调节及机制.方法采用免疫荧光和琼脂糖凝胶电泳技术检测小鼠单核巨噬细胞系J774A1中MIF的表达；用1 9/mL脂多糖(lipopolysaccharide,LPS)在体外建立J774A1细胞炎症反应模型,给予或不给予5 mol/L 15d-PGJ2预处理,采用RT-qPCR和Western blot技术检测其MIF mRNA和蛋白水平变化情况；使用过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor-γ,PPAR-γ)的药理学拮抗剂GW9662以探讨其能否逆转15d-PGJ2对MIF的调节作用；利用高内涵分析技术检测15d-PGJ2是否调节巨噬细胞炎症反应时PPAR-γ的核转位以探讨其是否通过PPAR-γ而调控MIF的表达.结果小鼠单核巨噬细胞系J774A.1在基因和蛋白水平均表达MIF；与模型组相比,15d-PGJ2预孵育组MIF mRNA和蛋白表达均显著下调(P＜0.05),且该作用被GW9662逆转；在J774A1炎症模型中,15d-PGJ2能够促进PPAR-γ入核.结论15d-PGJ2抑制单核巨噬细胞中MIF的表达且该作用可能依赖于PPAR-γ.
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