Enterovirulent E.coli,one of the major foodborne pathogens,contains more than 80 seROtypes classified into 6 groups including Enteropathogenic E.coli (EPEC),Enteroinvasive E.coli (EIEC) and Enterohaemorrhaai E.coli (EHEC).The objective of this work was to develop a rapid method for enterovirulent E.coli identification the basis of a methodology we proposed for the separation of intact bacterial cells by ion-exchange chromatography.