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Matrix Metalloprotease 2(MMP-2)is an important enzyme critical to extracellular matrix remodeling,especially cancer invasiveness,metastasis,and angiogenesis.Sensitive detection of MMP-2activity in vitro and on cellular levelis of great significance to medical diagnostics and prior treatment.Herein,we demonstrate an activatable T1MRI strategy by using Fe3O4-pep-Gd as NP-substrates for kinetic analysis of MMP-2.