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To demonstrate whether praline-rich tyrosine kinase-2(Pyk2)participate in chemokine receptor 7s(CCR7)downstream signal network,determine this molecules role and mechanism in chemokine receptor 7 regulating squamous cell carcinoma of the head and neck(SCCHN)viability and metastasis in vivo and vitro.Constructed the stable praline-rich tyrosine kinase-2 related non-kinase(PRNK)expressing SCCHN cells,examined the SCCHN cells viability,apoptosis,migration,invasion and adhension ability in transfected/or not cells.Constructed SCCHN tumor model in the nude mice and assay the tumor growth rate.Examined E-cadherin and Vimentin expression when Pyk2 inactivated.We successfully constructed the stable PRNK expressing SCCHN cells,and these cells exhibited the low viability,high apoptosis,low migration,low invasion and low adhension ability.In nude mice body,tumor formed by these cells also grow slowly compare to control group.When Pyk2 inactivated,the CCR7 induced E-cadherin and Vimentin expression were changed.Pyk2 is a key downstream signal molecules of CCR7 in SCCHN,which promotes SCCHN tumorigenesis and progression.