三种马铃薯Y病毒属病毒通用型单克隆抗体的鉴定

来源 :中国植物病理学会2016年学术年会 | 被引量 : 0次 | 上传用户:yinzheng1974
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  鉴定和分析马铃薯Y病毒属的三种病毒病,即大豆花叶病毒(Soybean mosaic vi-rus,SMV)、马铃薯Y病毒(Potato virus Y,PVY)、三叶草黄脉病毒(Clover yellow vein virus,CLYVV)的通用型单克隆抗体.提取PVY感染的马铃薯叶片和SMV感染的大豆叶片总RNA,RT-PCR克隆PVY和SMV的Coat protein (CP)基因,利用已有的CLYVV CP基因,构建原核表达载体pET28-PVY CP、pET28-SMV CP和pET28-CLYVV CP.
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