Objectives:To investigate the pharmacokinetics of paeonol microemulsion,microemulsion-based gels and marketed paeonol ointments by the skin-blood synchronous microdialysis coupled with LC/MS.Methods:The microdialysis systems were established by linear probes and concentric circles probes.In vivo recovery of paeonol in skin is 69.7±4.8% and in blood is 51.6±7.2%.The paeonol microemulsion,microemulsion based gels and marketed paeonol ointments were administrated to rats.PBS (pH7.4) served as perfused solution.