Objective Interferon-gamma (IFNγ), or type Ⅱ interferon, is a cytokine that is critical for tumor control and for innate and adaptive immunity against viral and intracellular bacterial infections (Schoenborn and Wil son, 2007 ; Hanzlikova et al., 2012).The IFNγalso plays an important role in the ageing process (McNerlan et al.,2002; Kim et al., 2009).IFNγ expression increases in senescent vascular endothelial cells, a process that leads to pro-inflammatory response and contributes to arteriosclerosis (Sikorski et al., 2011).To find out the enhancers of IFNγ gene and conduct activity comparison of IFNγ enhancer homologous sequences from six different organisms (mouse, rabbit, dog, human, panda and ox).