【摘 要】
：
为研究IGF-I基因对藏猪、雅南猪生长发育的影响,以GenBank中猪IGF-I基因序列(登录号:DQ121132)设计引物,提取藏猪、雅南猪肝脏组织总RNA,应用RT-PCR技术从藏猪和雅南猪肝脏克隆出IGF-I基因,分别将藏猪、雅南猪IGF-I基因克隆到PMD19-T载体上,构建重组质粒pMD19-T-IGF-I,经PCR鉴定、测序及分析得该基因大小为612bp,包含一个完整的ORF,该ORF
【机 构】
：
四川农业大学动物医学院动物微生态研究中心,四川成都611130 四川农业大学动物医学院动物微生态研
论文部分内容阅读
为研究IGF-I基因对藏猪、雅南猪生长发育的影响,以GenBank中猪IGF-I基因序列(登录号:DQ121132)设计引物,提取藏猪、雅南猪肝脏组织总RNA,应用RT-PCR技术从藏猪和雅南猪肝脏克隆出IGF-I基因,分别将藏猪、雅南猪IGF-I基因克隆到PMD19-T载体上,构建重组质粒pMD19-T-IGF-I,经PCR鉴定、测序及分析得该基因大小为612bp,包含一个完整的ORF,该ORF共有462核苷酸,编码153个氨基酸;所测得的藏猪和雅南猪基因序列与Muller等报道的猪IGF-I基因编码序列高度同源,比对序列发现,藏猪的同源性为100％,雅南猪的为99.61％;藏猪IGF-I与雅南猪IGF-I基因序列的同源性为99.61％;雅南猪在258bp处发生A-G突变,但其编码的氨基酸未发生改变.
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