【摘 要】
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As plant genomics rapidly develops with the use of high-throughput sequencing techniques,plant phenotyping lags far behind.To relieve this bottleneck,we have adopted a strategy by combining high-throu
【机 构】
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National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research,Huazh
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As plant genomics rapidly develops with the use of high-throughput sequencing techniques,plant phenotyping lags far behind.To relieve this bottleneck,we have adopted a strategy by combining high-throughput phenotyping and GWAS to unlock genetic information encoded in the rice genome for controlling the complex traits,which demonstrated the feasibility of replacing laborious manual phenotyping with objective,efficient,and non-destructive phenotyping tools such as HRPF.Based on a performance evaluation of the HRPF and GWAS results,we demonstrated that high-throughput phenotyping has the potential to replace traditional phenotyping techniques and can provide novel gene identification information.With appropriate modifications on the image analyses,we anticipate that the combination of HRPF and GWAS can be used in a wide spectrum of other plant species to determine their genetic architecture and provide insights into basic biological processes.
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