【摘 要】
：
We previously established and characterized two insect cell lines (BmN-SWU1 and BmN-SWU2) from Bombyx mori ovaries.Here,we examined their differential susceptibilities to Bombyx mori nucleopolyhedrovi
【机 构】
：
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China;Insti
论文部分内容阅读
We previously established and characterized two insect cell lines (BmN-SWU1 and BmN-SWU2) from Bombyx mori ovaries.Here,we examined their differential susceptibilities to Bombyx mori nucleopolyhedrovirus (BmNPV) despite having originated from the same tissue source.BmN-SWU1 cells were susceptible and supported high titers of BmNPV replication,while BmN-SWU2 cells were resistant to BmNPV infection.Subcellular localization analysis demonstrated that very few BmNPV particles could be imported into BmN-SWU2 cells.However,initiation of BmNPV DNA replication but not amplification was detected in BmN-SWU2 cells after transfection with vA4prm-VP39-EGFP bacmid DNA.BmNPV transcription assays showed that late and very late but not early viral genes apparently were blocked in BmNSWU2 cells by unknown mechanisms.Further syncytium formation assays demonstrated that the BmNPV envelope fusion protein GP64 could not mediate BmN-SWU2 host cell-cell membrane fusion.Taken together,these results indicate that these two cell lines represent optimal tools for investigating host-virus interactions and insect antiviral mechanisms.
其他文献
本研究将外源和本场两种种公猪精液应用于本场适配母猪，并对母猪繁殖表现、后代断奶和育肥成绩进行对比分析.研究结果表明，使用外源精液的母猪返情率低于对照组4％，但其产仔性能与对照组差异不显著(P＞0.05)；试验组仔猪窝均断奶数、窝均断奶重与对照组差异均不显著(P＞0.05)，但只均断奶重显著高于对照组20.95g (P＜0.05)；试验组母猪所产后代育肥期平均日增重、上市日龄比对照组极显著提高了40
猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)是引起世界各地猪急性肠炎并水样腹泻的重要病原之一,PEDV的S蛋白是由S基因编码的膜蛋白,S蛋白与细胞表面受体结合,诱导细胞膜融合,在感染宿主体内S蛋白介导抗体产生过程中发挥重要生物学作用.S蛋白表面含有重要的抗原决定簇,有良好的免疫原性.通过对PEDV流行毒株S基因进行序列分析,选取S1蛋白502-641
猪流行性腹泻(Porcine epidemic diarrhea,PED)是由猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)引起猪的一种高度接触性肠道传染病,其特征为呕吐、腹泻、脱水[1],各种日龄的猪均易感,以哺乳仔猪的发病率最高.PEDV的M基因编码M蛋白,M蛋白在病毒粒子的组装和出芽过程中具有重要作用.序列分析表明,PEDVM基因与其他冠状病毒的
The silkworm is an important economic insect and the viral disease,especially nucleopolyhedrovirus,results in a great loss of sericulture.Many host genes were confirmed to have the activity of nucleop
桑椹属浆果类保健水果,具有较高的食药用价值.但由于其表皮极薄,极易受微生物侵染,直接影响桑椹的食用价值.本研究从腐败的桑椹中分离出这些微生物并进行种属鉴定,为进一步开展桑椹保鲜技术提供依据.本试验室温下出现明显霉斑的桑椹为材料,分别用含纤维素和果胶的筛选培养基进行微生物筛选,经过分离纯化,从含纤维素培养基上筛选到一株细菌,命名为XX,和一株真菌,命名为XZ；从含果胶培养基上筛选出一株细菌,命名为G
Microsporidia are fungal-like unicellular eukaryotes which can develop as obligate intracellular parasites and infect a variety of hosts from protozoa to mammals.Recent reports suggest that the spore
Baculovirus late expression factors (LEFs) are involved in viral DNA replication and viral gene regulation.Baculovirus lef-11 gene encodes a putative protein with predicted molecular weight about 13KD
桑椹果实组织娇嫩多汁,表皮极薄,在采收过程中极易损伤、致腐菌侵染,导致果实变质.本研究试图从桑椹中分离能抑制致腐菌生长的拮抗微生物,为进一步开发成本低廉,操作简单,安全无害等生物保鲜技术提供支撑.试验以完好健康的桑椹为试材,采用浓度梯度稀释法筛选,分离纯化出数株酵母菌,以前期筛选到的桑椹致腐菌——核盘菌属和链格孢属为拮抗对象,进行平板对峙试验,筛选到一株对这两种致腐菌都有一定抗性的红色酵母菌,并进
采用先进的统计学方法对桑树内生拮抗菌Bacillus subtilis X-16 产抗真菌活性物质的发酵培养基进行了优化，以期得到最佳的培养基配方.以桑粘格孢菌为效价测定指示菌，以效价为指标，首先采用单次单因子试验筛选出培养基中的最佳缓效碳源、速效碳源、氮源，采用正交试验得出最佳的碳氮比(C/N)和无机盐，最后采用均匀设计试验，获得最佳培养基配方：玉米粉10.0 g、蔗糖10.0 g、酵母浸膏粉5
朱砂叶螨是世界性害螨且对包括炔螨特在内的许多常用杀螨剂产生了抗药性.本研究在室内采用炔螨特对朱砂叶螨敏感品系进行抗性选育,得到抗性倍数达37.78 倍的抗性品系(PRR34).通过与敏感品系比较,明确了抗炔螨特品系对7 中常用杀虫杀螨剂的交互抗性,通过特异性酶抑制剂和酶活测定初步明确谷胱甘肽S-转移酶参与了朱砂叶螨对炔螨特的抗性.克隆得到朱砂叶螨谷胱甘肽S-转移酶基因(TcGSTd1、TcGSTd