Laboratory samples of commercially available first-generation Chromolith columns are compared to their successor, the second-generation Chromolith columns.Plate height curve analysis allowed to determine the major reduction in the eddy dispersion and the mass transfer terms.Morphologies of the stationary phases are investigated and compared for both Chromolith generations.The porosity, pore volume, and pore size distributions are determined for these columns using nitrogen sorption and mercury intrusion porosimetry.Electrostatic exclusion chromatography is utilized to specify the overall, internal, and external porosities of the columns.Scanning electron microscopy images are acquired enabling a first estimation of the actual monolith morphology and structural differences, as well as conventional distance measurements of the macropore space and skeleton.