家蚕tret1-like因子的鉴定及表达分析

来源 :江苏省遗传学会2016年学术研讨会 | 被引量 : 0次 | 上传用户:lxy850628
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  家蚕tret1-like 基因对海藻糖转运蛋白具有一定调控作用,从而对细胞内海藻糖水平进行调控。本研究利用生物信息学方法从家蚕基因组数据库中鉴定出69 个该基因家族成员,发现这些基因在家蚕28 个连锁群上为不均衡分布,其中 27 号染色体上分布最多,有21 个家族成员,系统发育分析显示出这些基因分为3 个亚组。我们选取其中一个基因,分别在抗BmNPV 品种和常规品种中克隆出其CDS 序列。同时,对抗BmNPV家蚕品种及其对照品种经口接种BmNPV 病毒,取不同时间点脂肪体组织材料,RT-PCR检测发现,该基因在抗性品种添毒3h 后开始表达,随添毒时间的增长其表达量呈现先上调再下调,而在非抗品种中表达量呈相对平稳的趋势。
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