【摘 要】
：
实验室前期从灰葡萄孢T-DNA插入突变体库中筛选获得了一株致病力完全丧失的突变体BCt89,明确了其突变基因为BcPDR1,该基因编码一个功能未知的假定蛋白,无同源基因和保守
【机 构】
：
河北农业大学真菌毒素与植物分子病理学实验室保定071001
论文部分内容阅读
实验室前期从灰葡萄孢T-DNA插入突变体库中筛选获得了一株致病力完全丧失的突变体BCt89,明确了其突变基因为BcPDR1,该基因编码一个功能未知的假定蛋白,无同源基因和保守结构域。为进一步明确该基因的功能,本研究利用基因敲除技术,获得了该基因的敲除突变体△BcPDR1；对突变体BCt89和△BcPDR1的表型进行分析,发现突变体的生长速度减慢,不产生分生孢子和菌核,菌丝纤细、分隔短、颜色浅,表明BcPDR1基因参与灰葡萄孢的生长、发育过程。
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