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AIM: To investigate the regulation of key genes in the bile acid pathways by farnesoid X receptor(FXR)agonist,chenodeoxycholic acid(CDCA)in primary mouse hepatocytes.METHODS:C57BL/6 mouse hepatocytes were isolated by modified two-step collagenase perfusion method,purified by density gradient centrifugation,and seeded at the density of 1×106 cells/well in a 6-well culture dish.