Casein Kinase Ⅱ Pathway Inhibition Promotes Retinal Ganglion Cell Survival and axon regeneration via

来源 :第八届亚洲神经眼科大会暨第四届全国神经眼科学术会议 | 被引量 : 0次 | 上传用户:May-02
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Purpose: To characterize the roles of casein kinase Ⅱ (CK2) pathway in RGC survival and axon regeneration after optic nerve (ON)injury.Methods: Adult rats received ON transection and a peripheral nerve (PN) graft onto proximal end of the ON stump to provide a favourable environment for axon regrowth.Intravitreal injections of CK2 inhibitor TBB or DMAT were performed at 3, 9, and 15 days after ON surgery.Animals survived for 3 weeks.Three days before animal sacrifice,FluoroGold was injected into distal end of the PN graft to retrogradely label axon-regenerating RGCs.After counting FG-labled RGCs, retinas were double-immunostained with TUJ1 antibody to identify viable RGCs and ED1 antibody to reveal macrophages.CK2 activity assay was applied to the effectiveness in blocking the signal transduction of CK2 pathway of the 2 inhibitors.
其他文献
Objective: The aim of this study was to set up an ideal rat model for ocular ischemic syndrome (OIS) and to investigate whether abnormal activation of apoptosis signaling pathways related proteins pla
Objective: To report a case of neuritis like optic neuropathy accompanied with mtDNA G11778A mutation with long-term follow-up.Methods: Case report.Results: A boy of 10 years old presented with a comp
会议
Background: Mitochondrial optic neuropathy (MON) is a major-spectrum optic neuropathy resulting from genetic and drug-related causes.Their clinical manifestations are very similar which make puzzles i
Objective: To present a case of alveolar rhabdomyosarcoma (ARMS), unusual in its presentation as orbit apex-carvernous sinus apex syndrome and also its origin from the sphenoid sinus.Methods: A 23-yea
会议
PURPOSE: The purpose of this study is to determine the interocular differences in peripapillary retinal nerve fiber layer (RNFL) and peripapillary choroidal thickness (PCT)in healthy adults with isome
Background: To evaluate the efficacy of two botulinum toxin A injection patterns with or without the medial lower eyelid (MLE) in treating benign essential blepharospasm (BEB) and influencing lacrimal
Purpose: To characterize optic disc changes of Lebers hereditary optic neuropathy (LHON) by optical coherence tomography (OCT),Methods: 60 eyes from 60 consecutive LHON patients and 9 eyes from 9 age
会议
Objective To describe the characteristics of thickness of retinal and choroidal in peripapillary and macular region in patients with optic atrophy caused by NAION, and compare them with control subjec
会议
Objective To analyze the clinical characteristics and prognosis of acute encephalitis with visual impairment in children.Methods The clinical features including clinical manifestations, laboratory tes
会议
目的 本文系统评价了玻璃体内注射(VEGF)药物治疗糖尿病性黄斑水肿的有效性和安全性.方法 以抗血管内皮因子,糖尿病黄斑水肿,为关键词,在pubmed,EMBASE,Cochrane图书馆等进行文献检索,检索到文献762篇,按照文献等级等检索到的文献进行分类,纳入随机对照研究进行荟萃分析,以评价玻璃体内注射抗VEGF药物在减轻糖尿病性黄斑水肿和改善视力方面的作用效果总结并评价了上述治疗)的不良事件