【摘 要】
：
Embryonic stem (ES) cell-based therapy is a promising treatment for neurodegenerative diseases.Although it is well established that ES cells have the potential to differentiate into dopamine neurons,t
【机 构】
：
Baylor College of Medicine USA
论文部分内容阅读
Embryonic stem (ES) cell-based therapy is a promising treatment for neurodegenerative diseases.Although it is well established that ES cells have the potential to differentiate into dopamine neurons,the molecular basis of this process, particularly the role of microRNAs (miRNAs), remains largely unknown.Here we report that miR-132 plays a key role in the differentiation of dopamine neurons by directly regulating the expression of Nurr1.First, we constructed a mouse ES cell line CGRS,which stably expresses GFP under the tyrosine hydroxylase (TH)-promoter, so the TH-positive neurons could be easily sorted out by a fluorescence-activated cell sorter (FACS).Then, we performed a miRNA array analysis in the purified TH-positive neurons and we identified miR-132 to play an important role in the differentiation processing of TH-positive neurons through the direct regulation of Nurr1 gene.
其他文献
Cell-based therapies are expected to revolutionize clinical medicine in the same way as monoclonal antibodies did.Although the idea to use cells as pharmaceutical compounds is not new, the clinical ap
The seminar describes a strategy to engineer human cell surfaces via liposome fusion for applications in biophysical membrane dynamics and regenerative medicine.
We have developed novel technology "cell sheet-based tissue engineering", which has realized celldense tissue fabrication without any scaffolds.Confluently cultured cells are harvested as contiguous c
With respect to the limited lifespan of glutaraldehyde-treated bioprostheses to date there is almost no altemative when heart valve replacement surgery is required.Current research attempts to develop
Recently the scientific community has recognize the entity of tumor initiating cells which are responsible for the development of a tumor.Several mechanisms seem to be implicated in epigenetic and gen
Esophageal cancer is one of most common malignancies.Cancer stem cells (CSC) are considered to be resistant to chemotherapy and radiotherapy, which might be associated with the recurrence of the tumor
Bone implanted materials are needed to treating considerable disorders, including bone defects and nonunion resulting from bone tumor excision or trauma, and in procedures such as spinal and joint fus
Electrically conducting polymers induce specific cellular responses at the molecular level.One of the crucial limitations of the use of conducting polymers in tissue engineering is their inability to
We previously demonstrated in a clinical pilot study that CD34+ cells were the most appropriate for long-term cardiac regeneration after acute myocardial infarct (AMI), once directly re-injected into
Mesenchymal stem cells (MSCs) derived from either bone marrow (BMSCs) or placenta (PMSCs) have the capacity to suppress immune responses to mitogenic and allogeneic stimulations.Both cell contact and