Objective Explore the whole-tumor parameters based on the three-time point (3TP) method of dynamic contrast enhancement MRI (DCE-MRI) to quantitatively assess the functional vascularity of macroadenoama, and provide the optimal approach preoperation. Method Sixteen macroadenoma and 8 normal pituitary subjects with DCE-MRI images were recruited. Both of the tumors were confirmed by the pathology. All MRI examinations were performed on GE-HDxt 3.0T scanner with 8-channel head coil. The dynamic T1-weighted fast spin-echo sequence with the injection of gadolinium (1.0 mmol/kg) was performed coronally. Image analysis: Firstly, according to its enhancement time-signal curve (TIC), each voxel within 3D tumor was grouped to one in three types by a preset threshold of ±10%: washout (type1, < -10%), plateau (type2, -10%~10%) and persistently enhancement (type3, >10%) (Figure 1A); the volume percentage of each type was then calculated. Second, project the different colors into the original images. The parameters were analyzed by Mann-Whitney U test. A p<0.05 was considered to indicate a statistically significant difference. Results Significant difference was found in volume percentage of the type1 (p=0.014). Compared with normal group, the macroadenoma has less washout type (median 19.25 vs 37.32). From the examples of two groups, there was obviously difference color distribution in the tumor and normal pituitary. Conclusion The volume percentage of the washout type is significantly lower in the macroadenoma than it in normal pituitary, which suggested that lower expressed vascular endothelial growth factor (VEGF) may make a contribution to the functional vascularity changes. Since VEGF expression responsible for intratumoral hemorrhage, the blue area may have the high risk of hemorrhage and this may provide a more reliable and intuitive method to choose the optimal path presurgey.