【摘 要】
：
1,4,6-androstatriene-3,17-dione (ATD) is best aromatase inhibitor and increase bioavailable testosterone significantly higher (over 400%) than any other testosterone booster advertised as highly stren
【机 构】
：
Center for Environmental Technology Research, Korea Institute of Science and Technology, P.O.Box 131
【出 处】
：
DaLian Internationall Symposia and Exhibition on Chromatogra
论文部分内容阅读
1,4,6-androstatriene-3,17-dione (ATD) is best aromatase inhibitor and increase bioavailable testosterone significantly higher (over 400%) than any other testosterone booster advertised as highly strength.So, it advertised as highly anabolic/androgenic compound promoting muscularity, enhancing strength, and overall physical performance and available on the intemet and in the health stores.Urinary ATD and its metabolite following oral administration of ATD (dose 25 mg/tablet) to male volunteer were isolated from urine by way of PAD-1 adsorption, enzymatic hydrolysis with β-glucuronidase form E-coli and liquid/liquid extraction with n-hexane.The extracted ATD and its metabolite were compared to blank urine, spiked urine and urinary sample by LC/ESFTOF/MS.For confirmation, these were derivatized with MSTFA/NH4I/DTE (500∶4∶2 (w/w/w)) and detected by GC/MS/MS with chemical ionization and GC/TOF/MS.From these results, we suppose that the metabolite of ATD was hydroxylated.And we could detect the free ATD and glucuronidated ATD metabolite,and now work to excretion study.
其他文献
Supercritical (SC) fluid chromatography, mostly with carbon dioxide as a mobile phase, is used to measure the thermodynamic properties such as retention factor and diffusion coefficient for instance,
A novel approach of liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) for rapid analysis of very long chain fatty acids is described.By using a C18 column and a isocratic elu
Phospholipid molecular species have been determined in the spent dialysates of 21 patients on continuous ambulatory peritoneal dialysis (CAPD) by LC-ESI-MS.Glycerophosphoehtanolamine (PE), phosphatidy
Five naturally bioactive compounds, belonging to the representative flavonoid and pentacyclic triterpenoid types were separated by liquid chromatography (LC) and analyzed on-line with negative ion ele
Ultra high performance liquid chromatography (UPLC) employs 1.7 μm porous particles resulting in higher peak capacity, better resolution and sensitivity compared with traditional HPLC, and has been ap
For supercritical fluid chromatography (SFC), the mobile phase is generally of high diffusivity and low viscosity, which is achievable with the combination of a compressible fluid, usually carbon diox
Proteomics is a promising subject to discover potential biomarkers that could be used in the diagnosis and better understanding of human diseases.One of challenges in proteome study is the detection o
Large scale characterization of phosphoproteins requires highly specific methods for purification of phosphopeptides because of the low abundance of phosphoproteins and substoiehiometry of phosphoryla
Pyrolysis-gas chromatography (Py-GC) and liquid chromatography (LC) are widely used for the characterization of (co)polymers.The combination of organic SEC and Py-GC, either in the off-line mode or on
Present research describes a new sample preparation method termed directly suspended droplet liquid-liquid-liquid phase microextraction for determining residues of the analgesic drug diclofenac (2-[2-