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Esophageal squamous cell carcinoma (ESCC) patients always had a poor prognosis, because they were diagnosed at a later stage with distant or lymph node metastasis when seeking medicinal help.In this study, we evaluated chemopreventive efficacy of "Lianhua Shenjia" (LHSJ) formula, a Chinese herbal mixture of four plants (phillygenin, trichosanthin,Lupeol acetate from Cortex Periplocae and polysaccharides from Codonopsis Pilosula) on the development of 4-nitroquinoline-1-oxide (4NQO) induced ESCC in mice.