Fez1基因在调控乳腺癌细胞MCF-7增殖与细胞周期方面的作用

来源 :中华医学会第十四次全国妇科肿瘤学术会议 | 被引量 : 0次 | 上传用户:dhgczjd
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目的 现在已经有很多相关研究证实Fezl是一种抑癌基因,在多种肿瘤组织中表达较正常组织来说有不同程度的降低.在肿瘤细胞中将Fezl进行过表达之后能够使肿瘤细胞停留在G2/M期的细胞增加.本实验旨在研究Fezl基因在调控乳腺癌细胞系MCF-7生长与增殖中的作用.方法 构建Fezl-GFP质粒并将其与GFP质粒分别转染入MCF-7细胞中,通过流式细胞仪检测MCF-7细胞周期的变化.
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