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An error study of the electron beam spread experiment in the retarding field energy analyzer is discussed in this paper which is based on a K-band space travelling wave tube(TWT).The collector is coated with molybdenum on the copper upper substrate to validate whether the lower collector current is caused by secondary electrons.The study is meaningful to have a better resolution in the future experiment of the electron beam energy spread,and it will definitely be a reference for the design of multistage depressed collector and TWTs.