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Objective: To investigate the the correlation between gr/gr deletions and b2/b3 deletions in the AZFc region as well as DAZ gene copy deletions with male spermatogenic impairment.Methods: Exerting PCR,PCR-RFLP technology and different types of Y chromosome specific sequence tagged sites(STS) to analyze the gr/gr deletions and b2/b3 deletions in the AZFc regions well as DAZ gene copy deletions in 121 men with different spermatogenesis impairment and 95 donors from sperm bank.