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@@Progesterone is a steroidal hormone with a vital role in the maintenance of human and animal health. Its imbalance can cause malformations in the reproductive system as well as infertility problems. Progesterone is secreted by the uterus to prepare it for pregnancy and maintain the pregnancy after conception secretes progesterone. Therefore, progesterone is adequate to diagnose an early pregnancy. Progesterone levels should be at sufficiently high values in the organism to assure the embryo development. Thus, monitoring of progesterone levels is important to reproductive system. Here we describe a competitive immunoassay method for the determination of pregnancy by microchip electrophoresis (MCE) with laser induced fluorescence (LIF) detection. The method is based on the competitive immunoreaction between pregnancy and fluorescein-5-isothiocyanate (FITC) labeled pregnancy with a limited amount of antibody, and LIF detection of the fluorescent species after MCE separation of free FITC-labeled pregnancy from the immunocomplex. Under the optimized conditions, pregnancy was detected in a range of 0.8~80 nmol/L, and the detection limit (S/N = 3) was 0.4 nmol/L. This present tmethod has been applied to determine progesterone in the human serum samples from normal and pregnancy.