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Vitamins are a well-known group of compounds that are essential for human health and are classified into two main groups, water-soluble andfat-soluble.Reversed-phase HPLC is a well-suited technique for vitamin analysis.Commonly, water-soluble vitamins are determined using an aqueous mobile phase with low-organic solvent content, whereas fat-soluble vitamins are determined using mobile phases with high organic solvent contents.