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背景与目的应用RNA干扰联合基因芯片技术检测HMGB1表达下调后肺癌细胞L9981基因表达谱的差异情况,筛选与HMGB1相互作用的基因。方法采用阳离子脂质体试剂向人肺癌细胞L9981转染靶向HMGB1基因的siRNA,下调HMGB1的表达。应用Aymetrix HU133plus2基因芯片检测人肺癌细胞L9981HMGB1基因表达下调后,全基因表达谱的变化,并应用GCOS(Gene-Chip Operation Software)软件分析筛查数据,对相关基因进行综合分析。结果RNA干扰抑制人大细胞肺癌细胞株L9981HMGB1基因表达后,GCOS软件分析基因芯片分析其表达谱的变化。结果发现1433个探针表达明显改变,对应有明确名称的基因为879个,其中上调的基因有295个,下调的有584个;EST序列216个,其中上调96个,下调120个。结论肺癌细胞L9981HMGB1基因下调后,其细胞基因表达谱发生明显变化,确定表达差异基因879个,表明HMGB1基因表达受很多相关基因及信号通路的调控,与肺癌的侵袭、转移及肺癌形成等生物学过程密切相关。
BACKGROUND & AIM: To detect the gene expression profiles of L9981 in lung cancer cells after the down-regulation of HMGB1 expression by RNA interference and gene chip technology and to screen the genes that interact with HMGB1. Methods Human lung cancer cell line L9981 was transfected with siRNA targeted to HMGB1 gene and the expression of HMGB1 was down-regulated by cationic liposome reagent. The gene expression profile of human L9981HMGB1 gene was detected by using the gene of Aymetrix HU133plus2. The gene expression profile was analyzed by Gene-Chip Operation Software (GCOS) and the related genes were analyzed. Results RNAi inhibited the expression of L9981HMGB1 gene in human large cell lung cancer cell line, and analyzed by GCOS software. The results showed that the number of 1433 probes changed significantly, corresponding to 879 genes with a clear name, of which 295 genes were up-regulated and 584 genes were down-regulated. There were 216 EST sequences with 96 genes up-regulated and 120 down-regulated. Conclusion The down-regulated expression of L9981HMGB1 gene in lung cancer cells significantly changes the gene expression profiles of 879 cells, indicating that HMGB1 gene expression is regulated by many related genes and signaling pathways, which is closely associated with the invasion and metastasis of lung cancer and the development of lung cancer The process is closely related.