【摘 要】
：
An enhanced facile strategy through a green chemistry method for the surface modification of poly (dimethylsiloxane) (PDMS) was developed via in situ bulk free radical polymerization to resist nonspec
【机 构】
：
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,P.R.China;Key Lab
【出 处】
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中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
论文部分内容阅读
An enhanced facile strategy through a green chemistry method for the surface modification of poly (dimethylsiloxane) (PDMS) was developed via in situ bulk free radical polymerization to resist nonspecific protein adsorption and to reduce electroosmotic flow (EOF).After silanization by 3-methacryloxypropyltrimethoxysilane, allyl-polyethylene glycol (PEG) was conjugated onto PDMS surface with aid of sodium persulfate.The surface properties of PDMS were characterized with Fourier transform infrared absorption by attenuated total reflection (ATR-FTIR) and contact angle experiment.EOF measurements and nonspecific protein adsorption assay revealed stable EOF suppression and remarkable protein-repelling properties for more than 30 days.Separation of FITC-labeled amino acids demonstrated a high reproducibility (migration time, RSD 2.2%).Further separation of fluorescence-labeled proteins using PDMS microchips before and after surface modification showed that the PEG-modified PDMS microchip had an improved electrophoretic performance with a RSD of 1.49% for the migration time.In summary, a convenient strategy was developed for the modification of PDMS microchip to prevent nonspecific protein adsorption and to enhance electrophoretic performance.This improved environmentally friendly surface modification method will be useful for protein separation especially in batch production.
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