Patterning of nervous system requires precise input of the spatial cue.In our study, we found that the deneddylation complex CSN determine neural competence at a specific location by reading the temporal ecdysone signal cue.In CSN mutant, the non-innervated bristles at the posterior wing margin of Drosophila are transformed into neuron-innervated sensory organs.The transformation involves temporal up-regulation of ecdysone signaling that induces the target gene br during the prepupa-to-pupa transition.As a consequence, the gene product BR-Z1 activates Senseless (Sens) expression, which confers the neural competence to posterior wing margin bristles.We also found that the temporal suppression of br during prepupa-to pupa development requires the ecdysone receptor to switch from activator to repressor,which is mediated by the deneddylation activity of CSN and multiple cullins, the major substrates of CSN.Several CSN subunits associate with ecdysone receptors,and they acts in the same genetic pathway for temporal repression of br.Thus, our study indicates that the CSN integrates the temporal hormone signaling to endow a spatial neural suppressive activity in sensory bristle patterning.