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Four chiral copper(Ⅱ)complexes with Schiff-base ligands,[Cu(R-L1)2]EAC(1)and [Cu(S-L1)2]EAC(2),[Cu(R-L2)2]EAC(3)and [Cu(S-L2)2]EAC(4),(R/S-L1 =(R/S)-(1-naththyl)-salicylaldimine,R/S-L2 =(R/S)-(1-naththyl)-3-methoxysalicylaldimine,EAC = ethyl acetate)were synthesized to serve as artificial nucleases.