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Current wastewater treatment systems and technologies have been demonstrated to be unsustainable and discharge standards become more stringent, this place a big challenge to the urban wastewater management. The separated collection and treat-ment of urine has attracted considerable attention in the last few years, and it is seen as a viable option for enhancing the flexibility of wastewater treatment system. Lit-eratures propose to develop a sustainable system for densely populated urban areas based on the new concepts of urine separation, onsite treatment and in-sewer treat-ment technologies. Unlike the existing system that passively collects and treats sew-age at great technical difficulty and cost, the new system effectively separates urine at the source and transforms it onsite into nitrate/nitrite. Then, the generated ni-trate/nitrite is further converted into harmless nitrogen gas in the sewer where soluble organics are co-removed with little effort. Since urine contains 80％nitrogen and 40% phosphorus in domestic wastewater, but makes up less than one percent of the total wastewater volume, effectively treat-ment of source-separated urine can provide a more promising way to solve above problems. Source-separated urine treatment has been divided into seven main pur-pose treatment processes: hygienisation, volume reduction, P-recovery, N-recovery, stabilisation, nutrient removal and handling of micropollutants. This study focus on onsite treatment technology development: the nutrient removal with MBR. Experi-ments have been carried out to investigate the feasibility of source-separated urine treatment with MBR and the optimal operation parameters for the MBR system management. Landfill plant nitrifying sludge was inoculated with synthetic wastewater and urine mixture. Select influent urine dilution rate 0.33, and monitor the effluent con-centration after a long stable period. Without extra alkalinity adding, ammonium and COD removal rate can reach about 41% and 92%, respectively. Experiments have been done to evaluate five key operation parameters (dilution rate, SRT, pH, DO and alkalinity dosage) effect on the performance of MBR. Results reveal the optimal op-eration conditions are as follow:pH 7.0 to 7.5, DO 3mg/L, dilution rate 0.27, extra alkalinity 24g/L NaHCO3, SRT 30d.