【摘 要】
：
高效稳定的离体再生体系是开展遗传转化工作的前提。本试验以桃砧木毛桃(Prunus persica)的无菌试管苗为材料,在实验室已有的研究基础上,首先对叶片诱导出的愈伤组织进行石蜡
论文部分内容阅读
高效稳定的离体再生体系是开展遗传转化工作的前提。本试验以桃砧木毛桃(Prunus persica)的无菌试管苗为材料,在实验室已有的研究基础上,首先对叶片诱导出的愈伤组织进行石蜡切片观察,从细胞形态学上确定了可能具有胚性的愈伤组织形态；其次研究探讨了细胞分裂素CPPU对毛桃叶片愈伤组织诱导、保存和植株再生的影响。主要研究结果如下：1.适合毛桃无菌试管苗继代保存的6-BA浓度为0.2mg/L,在此浓度下继代保存能够减少试管苗玻璃化现象的发生。2.毛桃叶片植株再生时采用0.1mg/L CPPU的再生效果比采用2mg/L6-BA和3mg/L TDZ要好。并且当CPPU0.5mg/L结合NAA0.1mg/L时再生率最好,可高达16.67%。3.适合毛桃叶片愈伤组织诱导的培养基为：LP基本培养基+CPPU0.5mg/L+NAA0.5mg/L+蔗糖20.0g/L+琼脂粉6.0g/L,暗培养21d后转至光下培养。适合毛桃叶柄愈伤组织诱导的植物激素配比为：CPPU0.1mg/L结合NAA0.5mg/L。4.适合毛桃愈伤组织继代保存的植物激素配比为：CPPU0.1mg/L结合NAA0.5mg/L,每28d继代一次,持续暗培养保存。毛桃愈伤组织在含有TDZ1mg/L和NAA0.5mg/L的培养基上再生出了植株,再生率为3.33%。5.适合毛桃无菌试管苗生根的培养基为：1/2LP基本培养基+IBA1.0mg/L+NAA0.1mg/L+活性炭0.5g/L+蔗糖20.0g/L+琼脂6.8g/L。培养35d生根率可达77.78%,平均每棵试管苗生根条数为1.06条,平均根长为7.18cm。
其他文献
不结球白菜(Brassica campestris ssp.Chinensis)由于其营养丰富,口味独特,已成为世界性的蔬菜作物之一。随着不结球白菜栽培面积的增加,诸多不利因素(如生物胁迫和非生物胁
The control of underactuated mechanical systems is very complex for the loss of its control inputs. The model of underactuated mechanical systems in a potential
Morphine is postulated a risk factor in promoting tumor growth and metastasis during the preoperative period,and high glycolysis of tumor cells is proved to acc
Stress is a potent risk factor for depression.Chronic stress can exacerbate and induce symptoms of depression.Clinical studies suggested that depressive patient
Lung cancer,predominantly non-small cell lung cancer (NSCLC),is a deadly disease with a 5-year survival rate of ＜15％.A number of environmental and genetic risk fa
本试验以6生椪柑/枳砧(Citrus reticulata)和20年生国庆一号温州蜜柑/枳砧(C. unshiu)为材料,研究了地表覆膜对两个品种果实汁胞中糖酸和6种矿质元素含量的影响。结果表明,覆
本试验选取2种广泛种植的西瓜品种“早佳（8424）”和“京欣二号”为试材，栽培于硒浓度分别为0、0.3、0.6、0.9、1.2mg·kg-1的基质中，待西瓜幼苗张到三叶一心时，移至培养箱对其进行
MicroRNAs (miRNAs) are some short RNAs that regulate multiple biological functions at post-transcriptional levels,such as tumorigenic processes,inflammatory les
Chondrocyte is involved in the destruction of joints in osteoarthritis (OA) patients.The aim of this study was to explore the expression level of small nucleola
红叶桃春季新叶初发时叶色红艳,在夏季高温下会出现“返青”现象,且随着叶龄的增加,基部叶片叶色逐渐由暗红转绿,花色素苷含量下降。与之相反,许多早熟桃品种为夏秋季红叶型,