【摘 要】
:
高效稳定的离体再生体系是开展遗传转化工作的前提。本试验以桃砧木毛桃(Prunus persica)的无菌试管苗为材料,在实验室已有的研究基础上,首先对叶片诱导出的愈伤组织进行石蜡
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高效稳定的离体再生体系是开展遗传转化工作的前提。本试验以桃砧木毛桃(Prunus persica)的无菌试管苗为材料,在实验室已有的研究基础上,首先对叶片诱导出的愈伤组织进行石蜡切片观察,从细胞形态学上确定了可能具有胚性的愈伤组织形态;其次研究探讨了细胞分裂素CPPU对毛桃叶片愈伤组织诱导、保存和植株再生的影响。主要研究结果如下:1.适合毛桃无菌试管苗继代保存的6-BA浓度为0.2mg/L,在此浓度下继代保存能够减少试管苗玻璃化现象的发生。2.毛桃叶片植株再生时采用0.1mg/L CPPU的再生效果比采用2mg/L6-BA和3mg/L TDZ要好。并且当CPPU0.5mg/L结合NAA0.1mg/L时再生率最好,可高达16.67%。3.适合毛桃叶片愈伤组织诱导的培养基为:LP基本培养基+CPPU0.5mg/L+NAA0.5mg/L+蔗糖20.0g/L+琼脂粉6.0g/L,暗培养21d后转至光下培养。适合毛桃叶柄愈伤组织诱导的植物激素配比为:CPPU0.1mg/L结合NAA0.5mg/L。4.适合毛桃愈伤组织继代保存的植物激素配比为:CPPU0.1mg/L结合NAA0.5mg/L,每28d继代一次,持续暗培养保存。毛桃愈伤组织在含有TDZ1mg/L和NAA0.5mg/L的培养基上再生出了植株,再生率为3.33%。5.适合毛桃无菌试管苗生根的培养基为:1/2LP基本培养基+IBA1.0mg/L+NAA0.1mg/L+活性炭0.5g/L+蔗糖20.0g/L+琼脂6.8g/L。培养35d生根率可达77.78%,平均每棵试管苗生根条数为1.06条,平均根长为7.18cm。
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