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A rapidly growing area of genome research is the generation of expressed sequence tags(ESTs)in which large numbers of4704clones were randomly selected from rice stem cDNA library and then they were sequence.The collection of ESTs reflects the level and complexity of gene expression in thespecific tissue.A total of2860ESTs were generated from stem tissue of young rice5-6leaf period and only1129ESTs were categorized into18groups categories of biological roles according to MIPS.The two largest categories of ESTs according with each role were metabolism and energy with18.95%and16.03%,respectively.Clustering and assembly of these ESTs resulted in a total of2235(TUT)with1912singleton and323contigs ESTs.Results of the EST data show17highly expressed genes represent by contigs composed of6or more ESTs,where the most abundant contig was Chlorophyll a-b binding protein2,chloroplast precursor with43ESTs similarity to Oryza sativa and following with tubullin alpha-3chain from Hordeum vulgare,homology to Oryza safiva with10ESTs.cDNA microarray of stem rice were using for detect high genes expression profiles induced by specific probes of phytohormone gibberellin en different time(12and36h).The result of array after hybridization show three genes induced by gibberellin in36h that may be play role in stem elongation and four genes related with a photosynthetic process and a large group of genes related with protein synthesis and metabolism were also expressed in this array.All the EST data are available on the Internet(www.estarray.org).