基于功能化导电聚合物的漆酶电极的构建及其直接电化学研究

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漆酶(EC 1.10.3.2)是含有多个活性中心(铜离子)的酶,在生物传感器的制备、燃料电池的研制等领域具有非常重要的应用价值。为获得高性能的漆酶电极,必须缩短漆酶活性位点与电极表面的间距,减小漆酶异相电子转移对漆酶取向的依赖。为实现上述目标必须发展新的固载方法。目前报道的有化学亲和策略,漆酶重组改造策略等。相比于漆酶的化学或分子生物学修饰,构建适宜于多活性中心漆酶异相电子转移的纳米结构载体是一条捷径。利用导电聚合物制备漆酶阴极的方法已有尝试,但存在漆酶上载量低等主要问题。而利用苯硼酸类功能导电聚合
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